A very nutritious general-purpose medium based on Columbia Agar Base enriched with 5% Sheep Blood, suitable for the isolation of most organisms including most fastidious anaerobes of clinical significance. Many workers claim that β-haemolysis is more readily apparent, particularly in group A streptococci, when Sheep Blood is used in place of Horse Blood.
Columbia Agar Base with 5% Defibrinated Sheep Blood
|Shelf Life (days)|
|Storage Temp (°C)|
|Bacteroides fragilis||NCTC 9343||Small grey colonies|
|Escherichia coli||NCTC 12241||Grey colonies|
|Streptococcus pneumoniae||NCTC 12977||Grey / green colonies with α-haemolysis|
|Streptococcus pyogenes||NCTC 12696||White colonies with β-haemolysis|