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This is a non-selective medium for the maintenance of Neisseria gonorrhoeae cultures. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae. NB: This is a Basic medium only and DOES NOT contain any selective agents. It is therefore not recommended for use as a primary isolation medium. -
There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of VCNT (Vancomycin, Colistin, Nystatin & Trimethoprim). Vancomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Nystatin to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.
