Incubate plates at 35-37°C in a humid atmosphere at 5-10% CO2 for 24-48 hours

Vancomycin. Colistin, Amphotericin, Trimehtoprim (V.C.A.T) E&O Laboratories Ltd VCAT Selective Supplement (LS0002) is an antibiotic supplement used to enhance the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.