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An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
A very nutritious isotonic general-purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. This particular formulation also has added Glycerol to act as cryopreservative if the medium is used for the long term frozen storage of microorganisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications. -
This is one of a number of selective enrichment broths that can be used in the isolation of Campylobacter spp from clinical, food and environmental specimens and contains nutrients to aid in the resuscitation of damaged organisms. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Vancomycin, Cefoperazone, Trimethoprim and Amphotericin B. Following incubation at 37ºC the broth is usually sub-cultured onto an appropriate solid Campylobacter medium. -
Dey & Engley Broth has the ability to neutralise antimicrobial chemicals and is used for environmental sampling for the detection and enumeration of microorganisms present in cosmetic and/or environmental samples. In this formulation the broth has been modified by the addition of Tween 80 (Polysorbate 80), Lecithin, Sodium thioglycollate, Sodium thiosulphate and Sodium bisulphite. It is recommended for use in environmental testing, particularly in areas subjected to surface disinfection, and conforms to the requirements of the British Pharmacopeia for sterility testing of appropriate pharmaceutical products. The product also conforms to ISO 21149 to act as a diluent in the method for the enumeration of aerobic bacteria from cosmetic samples. Lecithin and Polysorbate 80 (Tween 80) inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). Sodium thioglycollate neutralises mercurials, Sodium thiosulphate neutralises iodine and chlorine and Sodium bisulphite neutralises aldehydes. Bromocresol purple is incorporated into the medium to indicate glucose utilisation. -
A medium for the selective isolation of group B streptococci. This information has been obtained directly from the HPA files at the customers request. -
LIM Broth with 10% Serum A nutritious, selective broth medium utilising the base formulation developed by Todd and Hewitt for the enrichment of Group B Streptococci. The LIM broth is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of other bacteria. Serum is also included to enhance the nutritional qualities of the base medium.
