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Columbia Agar is a nutritious general-purpose basal medium capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. However when further enriched with Sterile Blood, which can be “chocolated” if required, the medium is generally used for the isolation of most clinically significant pathogens. The medium can be made selective for various groups of organisms by the addition of a range of antimicrobial supplements. This formulation complies with the Harmonized USP/EP/JP. -
A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 60°C. Suitable for the cultivation of most pathogens including many fastidious organisms and is particularly suitable for Haemophilus and Neisseria spp. -
A medium for the selective isolation of group B streptococci. This information has been obtained directly from the HPA files at the customers request. -
For in vitro diagnostic use only. SW0005-M055-3 Strep B Swab Kit contains Lim Broth, a nutritious medium for the enrichment and transport of Group B streptococci along with a sterile swab suitable for collection of patient samples. The product may be used to preserve, transport, and enrich Group B streptococci that may be present in samples obtained from patients by use of the swab. Each pack also contains a sterile swab in an individual peel-pouch. The swab has a polystyrene shaft with a small viscose tip. The shaft has a breakpoint 78mm from the tip allowing the shortened swab to engage in the shaft capture device in the lid and ensure that the swab is removed from the tube when the cap is unscrewed. Lim broth is a nutritious, selective broth utilising the base formulation developed by Todd and Hewitt (1) for the enrichment of Group B streptococci. It is intended as a selective, enrichment broth for Streptococcus agalactiae that may be present is specimens. The device is primarily intended for use in screening pregnant women for the presence of S. agalactiae, which poses a significant risk of infection in new-born infants that may result in serious illness or death. -
BM0016 micrAlert Coliforms is a selective medium for the detection, recovery, and isolation of coliforms from cleaned, disinfected inanimate surfaces. It may be used in conjunction with SW0006 micrAlert Neutraliser Swab Kit. Coliforms are facultatively anaerobic, Gram-negative bacteria that belong within the Enterobacteriaceae family. They are oxidase-negative, can ferment and produce gas from lactose when grown at 37°C, and express β-galactosidase activity. Escherichia coli can be further identified by producing growth at 44°C and indole from tryptophan. BM0016 micrAlert Coliforms is based on Minerals Modified Glutamate Medium, intended for enumerating coliforms from water samples. A medium containing glutamic acid was devised by Folpmers in 1948 (1) then further developed by Gray (2) in 1959 as a potential replacement for MacConkey Broth. In 1964, Gray then further modified the media to incorporate a variety of minerals to allow better lactose fermentation by coliforms and Escherichia coli (3). References 1. Folpmers, T. (1948) Is it justified to use Lactose Broth for the detection of Bact. coli in the presumptive test of routine water analysis. Leeuwenhoek ned. Tijdschr. 14: 58. 2. Gray, R.D. (1959) Formate lactose glutamate: a chemically defined medium as a possible substitute for MacConkey Broth in the presumptive coliform examination of water. J. Hyg. Camb., 57(3):249-265. 3. Gray, R.D. (1964) An improved formate lactose glutamate medium for the detection of Escherichia coli and other coliform organisms in water. J Hyg (Lond). 62(4):495-508. -
BM0014 micrAlert Listeria is a selective medium for the detection, recovery, and isolation of Listeria species from cleaned, disinfected inanimate surfaces. It should be used in conjunction with SW0006 micrAlert Neutraliser Swab Kit. BM0014 micrAlert Listeria is based on Half Fraser Broth, developed in 1988 by Fraser (1). Listeria monocytogenes is commonly found in soil, sewage, or faeces. It is difficult to eradicate and can cause serious food poisoning. L. monocytogenes is frequently tested for in food processing facilities to avoid contamination. Contamination can occur at all steps of the food manufacturing chain from raw materials to place of consumption. Confirmation that food processing surfaces are free from contamination with Listeria spp. after cleaning and disinfection is a critical step in preventing disease. Residual disinfectant actives may inhibit the growth of viable organisms preventing detection, so the neutralising agents contained in SW0006 micrAlert Neutraliser inactivate these residual disinfectants allowing improved recovery of sub-lethally damaged target organisms in BM0014 micrAlert Listeria. -
SW0006 micrAlert Neutraliser Swab Kit is a general-purpose neutralising buffer for use with micrAlert bacterial indicator products. The product is designed to neutralise the antimicrobial effects of disinfectants (1) allowing recovery of microorganisms sampled from surfaces that may contain disinfectant residues. The accompanying sterile swab is present to allow easy sampling of the target surface. This is a general-purpose, ready-to-use neutralising solution containing lecithin, polysorbate 80, histidine and sodium thiosulphate to inactivate disinfectants including hypochlorite bleaches, peroxy compounds, quaternary ammonium compounds, phenolics, alcohols, hexachlorophene, mercury products, halogens, and aldehydes (1). References 1. Russell AD. Neutralization procedures in the evaluation of bactericidal activity. In: Collins CH, Allwood MC, Bloomfield SF, Fox A, eds. (1981) Disinfectants: their use and evaluation of effectiveness. London: Academic Press, pp. 45-59. Tryptone is present to assist in recovery of non-lethally damaged organisms that may be present, sodium chloride provides osmotic balance, and pH is maintained by the phosphate buffer system. The sterile swab included in the kit allows for specimen collection from inanimate surfaces. -
BM0015 micrAlert Salmonella is a selective enrichment media for the isolation of Salmonella spp. from cleaned, disinfected inanimate surfaces. It should be used in conjunction with SW0006 micrAlert Neutraliser Swab Kit. BM0015 micrAlert Salmonella is designed to indicate the presence of Salmonella spp. on previously cleaned and disinfected surfaces. Salmonella spp. are frequently tested for on food processing surfaces to ensure the effectiveness of the cleaning/disinfection regimen and reduce the risk of food contamination. Salmonellae can decarboxylate lysine resulting in production of cadaverine, an alkaline amine capable of overcoming acid produced by fermentation thus maintaining a higher pH in the medium. Residual disinfectant actives may inhibit the growth of viable organisms preventing detection, so the neutralising agents contained in SW0006 micrAlert Neutraliser are designed to inactivate these residual disinfectants allowing improved recovery of sub-lethally damaged target organisms using BM0015 micrAlert Salmonella. -
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim. It is sometimes used in conjunction with Mueller-Hinton Agar. -
This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar. -
This is an aqueous solution of Oxalic acid (5%) suitable for use in the digestion and neutralisation of Sputum that may be contaminated with Pseudomonas like organisms prior to culture. -
This is one of the large group of media, affectionately known as ‘Peptone Water Sugars’, that are generally used in the screening and/or identification of organisms particularly the enterobacteriacae. A positive fermentation of the substrate is clearly indicated by the medium turning pink due to the inclusion Andrade’s Indicator. -
Saline (0.45%) is suitable for use in preparation of food samples and/or as a rinse fluid during examination of equipment etc. prior to culture. It can also be used as a general purpose diluent in many areas of the laboratory.
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BM1673 Virus Transport Medium (VTM) is a balanced medium for the transportation of viral samples (with or without a swab). The inclusion of Hanks balanced salt solution produces an isotonic medium with buffer capacity to maintain pH, provide essential mineral salts, and a visual pH indicator. Bovine serum albumin is present to help stabilise virus particles. Gentamicin & amphotericin B are added to prevent growth of bacteria and fungi that may be present in the specimen. • 3ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Non-hazardous • Storage: 2-25°C • Shelf life: 365 days • Diagnostic processing should commence within 72hrs of sample collection. Medium is suitable for analysis via molecular (eg PCR), cell culture and immunofluorescence testing procedures.
