Columbia Agar Base with 5% Defibrinated Sheep Blood

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Columbia Agar Base with 5% Defibrinated Sheep Blood

A very nutritious general-purpose medium based on Columbia Agar Base enriched with 5% Sheep Blood, suitable for the isolation of most organisms including most fastidious anaerobes of clinical significance. Many workers claim that β-haemolysis is more readily apparent, particularly in group A streptococci, when Sheep Blood is used in place of Horse Blood.

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Organisms Ref. No Result
Bacteroides fragilis NCTC 9343 Small grey colonies
Escherichia coli NCTC 12241 Grey colonies
Streptococcus pneumoniae NCTC 12977 Grey / green colonies with α-haemolysis
Streptococcus pyogenes NCTC 12696 White colonies with β-haemolysis