PP0070 Legionella BMPA Agar is a selective medium for the isolation of Legionella spp. used primarily in clinical and environmental laboratories. The medium contains yeast extract as a source of nitrogen, carbon and vitamins. Charcoal is also included to neutralise growth-inhibiting substances; ACES buffer/potassium hydroxide is added to maintain the optimal pH of 6.9 for growth of Legionella species. The medium includes iron (III) pyrophosphate as a source of iron, L-cysteine as an essential amino acid for the growth of Legionella species and α-ketoglutaric acid, which acts as a growth stimulant. The medium is made selective by the addition of cefamandole and polymyxin to inhibit most Gram-positive and Gram-negative organisms. Anisomycin is included to inhibit yeasts and fungi.
PP0070 is recommended by the UK Standards for Microbiology Investigations and tested in accordance with ISO 11133:2014.
