Primary UTI Agar

Primary UTI Agar

KM0007 Primary UTI Agar is a chromogenic agar that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections.

Urinary Tract Infections (UTI’s) account for 35-40% of all hospital acquired infections in the UK. Gram negative aerobic bacteria are responsible for a considerable proportion of UTI’s with Escherichia coli isolation rates at 80-90% of first-time infections. Various other opportunistic bacterial species can cause UTI’s.

KM0007 may be used as a chromogenic medium for the quantification and presumptive identification of bacteria in urine from clinical samples in line with the UK Standards for Microbiology Investigations and tested in accordance with the principles of ISO 11133:2014.

Related Supplements :
SHS500 Sterile Horse Serum 500ml

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated pH

Prepared pH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth: Cream colonies
Escherichia coli NCTC 12241 Growth: Purple colonies
Enterobacter aerogenes NCTC 10006 Growth: Metallic blue colonies
Proteus mirabilis NCTC 10975 Growth: Colourless colonies with brown halo
Enterococcus faecalis NCTC 12697 Growth: Blue/turquoise colonies
Streptococcus agalactiae ATCC 12386 Growth: Pale turquoise colonies
Salmonella typhimurium NCTC 12023 Growth: Colourless colonies
Pseudomonas aeruginosa NCTC 12903 Growth: Colourless colonies
Klebsiella pneumoniae NCTC 9633 Growth: Metallic blue colonies

 

Formula*

  g/L
Peptone 20.5
Chormogenic mix 4.0
Agar 12.5
Total 37.0
*Adjusted/supplemented as required to meet performance requirements  

Preparation

Suspend 37g/L in 980ml of deionised/purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium.

Autoclave to sterilise at 121°C for 15 minutes before cooling in a water bath to 45-50°C.

Aseptically add 20ml of sterile horse serum (E&O SHS).

Gently homogenize the final medium before aseptically dispensing the specified volume into appropriate sterile containers and allowing to cool.