KM0044 – Campylobacter Agar Base

//KM0044 – Campylobacter Agar Base

KM0044 – Campylobacter Agar Base

This is one of several selective media available for the isolation of Campylobacter spp. in clinical, food and environmental laboratories. Campylobacter agar base is based on the formulation from Bolton and Robertson.
The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium.
The medium is enriched with lysed horse blood and made selective by the addition of cefoperazone, to suppress other enteric organisms, and amphotericin to suppress yeast and fungal growth (Preston supplement LS0010).

Related Supplements :
LS0009 Campylobacter (Skirrow) Selective Supplement, LS0010 Campylobacter (Preston) Selective Supplement, Lysed Blood

SKU: KM0044 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Campylobacter jejuni NCTC 11322 Growth, grey colonies
Campylobacter coli ATCC 43478 Growth, grey colonies
Escherichia coli ATCC 25922 Inhibited
Proteus mirabilis ATCC 29906 Inhibited

 

Formula*

g/L
Peptone 20.0
Sodium chloride 5.0
Agar 12.0
Total 37.0
*Adjusted/supplement as required to meet performance requirements

Preparation

Suspend 37g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically add 70 ml/l of lysed horse blood and 2 vials/l of Preston supplement LS0010. Mix well and dispense into appropriate sterile containers.