Nutrient Broth No.2

//Nutrient Broth No.2

Nutrient Broth No.2

SKU: KM0041 Category:

Nutrient Broth No.2 is used for the cultivation of fastidious pathogens and other microorganisms. This general use medium, rich in nutrients, allows the growth of bacteria when there is a low level of cells.
The medium is particularly suitable as a secondary growth medium for staphylococci to be tested for coagulase production and also be used for sterility testing of aerobic organisms.
Beef extract and peptone provide the required carbon, nitrogen and vitamins. Sodium chloride maintains osmotic balance.


Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth
Escherichia coli NCTC 12241 Growth



Beef extract 10.0
Peptone 10.0
Sodium chloride 5.0
Total 25.0
*Adjusted/supplemented as required to meet performance requirements


Suspend 25g of the medium in one litre of deionised / purified water. Allow the medium to dissolve whilst mixing for 10 minutes. Dispense the required volume into the final containers and sterilise at 121°C for 15 minutes.