KM0025 – Tryptone Soya Broth

//KM0025 – Tryptone Soya Broth

KM0025 – Tryptone Soya Broth

A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms.
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).

 

SKU: KM0025 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Staphylococcus aureus (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 6538 Growth
Escherichia coli (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 8739 Growth
Bacillus subtilis (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 6633 Growth
Pseudomonoas aeruginosa (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 9027 Growth
Candida albicans (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 10231 Growth
Aspergillus brasiliensis (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 16404 Growth

 

Formula*

g/L
Tryptone 17.0
Soy peptone 3.0
Sodium chloride 5.0
Glucose 2.5
Di potassium hydrogen phosphate 2.5
Total 30.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 30g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.
Dispense into appropriate containers. Sterilise at 121°C for 15
minutes then cool to 45-50°C.