KM0038 – Brilliant Green Agar

//KM0038 – Brilliant Green Agar

KM0038 – Brilliant Green Agar

Brilliant green agar is for the selective isolation of Salmonella spp., other than S. typhi. Brilliant green agar was first cited by Kristensen et al. in 1925 but was subsequently modified by the Netherlands Institute for Public Health. Brilliant green agar is generally used in conjunction with XLD agar (KM0013) as a secondary plating medium for subculture from selective enrichment media in food and environmental testing. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella spp. from samples where the numbers may be low.
Beef extract, peptone and yeast extract provide the required carbon, nitrogen and vitamins. Di-sodium hydrogen phosphate and sodium di-hydrogen phosphate are buffering agents. Lactose and sucrose are fermentable carbohydrates. Phenol red is a pH indicator and turns the medium yellow during lactose and/or sucrose fermentation. Brilliant green inhibits Gram-positive bacteria and Gram-negative bacilli other than Salmonella spp.
NB: It is not recommended that this medium be used for the isolation of Salmonella typhi or Shigella spp.

Related Supplements :
LS0024 Novobiocin Supplement (20mgs/L)

SKU: KM0038 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Salmonella typhimurium NCTC 12023 Growth, red colonies
Salmonella enteritidis ATCC 13076 Growth, red colonies
Escherichia coli NCTC 12241 Inhibited
Enterococcus faecalis NCTC 12697 Inhibited

 

Formula*

g/L
Beef extract 5.0
Peptone 10.0
Yeast extract 3.0
Di-sodium hydrogen phosphate 1.0
Sodium di-hydrogen phosphate 0.6
Lactose 10.0
Sucrose 10.0
Phenol red 0.09
Brilliant green 0.0047
Agar 12.0
Total 52.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 52g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. DO NOT AUTOCLAVE this medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.