KM0015 – Legionella Agar

//KM0015 – Legionella Agar

KM0015 – Legionella Agar

Legionella agar was designed for the isolation of Legionella spp. and is used primarily in water and environmental laboratories. Since Legionella spp. are fastidious organisms, the medium contains yeast extract as a source of nitrogen, carbon and vitamins. Charcoal is also included to neutralise growth-inhibiting substances and agar is the solidifying agent.

The medium requires supplementation with ferric pyrophosphate as a source of iron, L-cysteine, an essential amino acid for the growth of Legionella spp. and α-ketoglutaric acid, which acts as a growth stimulant. ACES buffer/potassium hydroxide is also added to maintain the optimal pH of 6.9 for growth of Legionella spp. Omission of the L-cysteine produces a confirmation medium that can be used to test presumptive Legionella spp. as isolates will not be able to grow.

Selective versions of the medium can also be created by the addition of various selective agents. GVPC is the most popular for water testing and BMPA for clinical testing.

Related Supplements :
LS7044 Legionella BCYE Supplement, LS7045 BCYE without Cysteine

SKU: KM0015 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Legionella pneumophila NCTC 11192 Growth, white colonies

 

Formula*

g/L
Yeast extract 10.0
Charcoal 2.0
Agar 15.0
Total 27.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 27g of the medium in 950 ml of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and add the required supplements, allow to mix and aseptically dispense into appropriate sterile containers.
NB: Frequently mix the medium during dispensing to ensure the charcoal is kept in suspension.