KM0024 – Tryptone Soya Agar

//KM0024 – Tryptone Soya Agar

KM0024 – Tryptone Soya Agar

Tryptone Soya Agar (TSA) is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms. This medium meets the requirements of the Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. TSA is commonly referred to as Soybean-Casein Digest Agar. TSA supplemented with lecithin and Tween 80® is widely used in environmental monitoring.
With further enrichment using 5-10% sheep or horse blood, most fastidious organisms can be isolated and their haemolytic reactions can be determined in order to aid identification. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used.
The tryptone and soy peptone are the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium.

Related Supplements :
Defibrinated Sheep Blood, Defibrinated Horse Blood

SKU: KM0024 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Staphylococcus aureus (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 6538 Growth
Escherichia coli (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 8739 Growth
Bacillus subtilis (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 6633 Growth
Pseudomonas aeruginosa (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 9027 Growth
Candida albicans (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 10231 Growth
Aspergillus brasiliensis (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 16404 Growth
Streptococcus pneumoniae (with 5% defibrinated sheep blood) NCTC 12977 Growth, α-haemolysis
Streptococcus pyogenes (with 5% defibrinated sheep blood) NCTC 12696 Growth, β-haemolysis
Staphylococcus aureus (with 5% defibrinated sheep blood) NCTC 12981 Growth, β-haemolysis
Escherichia coli (with 5% defibrinated sheep blood) NCTC 12241 Growth, β-haemolysis

 

Formula*

g/L
Tryptone 17.0
Soy Peptone 3.0
Sodium chloride 5.0
Agar 15.0
Total 40.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes to completely dissolve the medium. Cool to 42-46°C. If required prepare 5-10% TSA Blood Agar by aseptically adding the appropriate volume of sterile defibrinated horse or sheep blood. Various selective supplements may also be aseptically added at this point to create a variety of selective blood agar products. Gently homogenize the final medium and dispense into appropriate sterile containers.