KM0048 – Malt Extract Agar

//KM0048 – Malt Extract Agar

KM0048 – Malt Extract Agar

Malt extract agar is used for the detection, isolation and enumeration yeasts and moulds. The high carbohydrate content of the medium ensures rapid growth of yeasts and moulds.
Malt extract and mycological peptone provide the carbon, protein and nutrient sources required by the organisms. The acidic nature of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth.
Malt extract agar can also be used for the cultivation of fungi, although with the prolonged incubation necessary, cultures may become overgrown by bacteria. The selectivity of the medium can be increased by lowering the pH to 3.5-4.0 or by the addition of selective agents such as chloramphenicol.

Related Supplements :
LS0050 Chloramphenicol Selective Supplement

SKU: KM0048 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Candida albicans NCPF 3179 Growth, white colonies
Aspergillus brasiliensis NCPF 2275 Growth, white mycelia with black spores

 

Formula*

g/L
Malt extract 30.0
Mycological peptone 5.0
Agar 15.0
Total 50.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 50g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 115°C for 10 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.

NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.