Blood Agar Base No.2

//Blood Agar Base No.2

Blood Agar Base No.2

SKU: KM0034 Category:

Blood agar base No. 2 is a general-purpose medium enriched with various concentrations of horse or sheep blood and is suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance.
Haemolysis observations may vary with the type of blood being used. Previous studies have shown that sheep blood provides the most reliable colony and haemolysis characteristics.
The peptone, yeast extract and liver digest act as nitrogen, carbon and vitamin sources in this medium. Sodium chloride maintains osmotic balance.

Related Supplements :
Defibrinated Sheep Blood, Defibrinated Horse Blood, LS0008 Staph/Strep Selective Supplement, LS0017 Neomycin Selective Supplement

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Streptococcus pneumoniae ATCC 6305 Growth, grey colonies with α-haemolysis
Escherichia coli ATCC 25922 Growth, opaque / grey colonies
Streptococcus pyogenes ATCC 19615 Growth, white colonies with β-haemolysis
Staphylococcus aureus ATCC 25923 Growth, white colonies



Peptone 15.0
Yeast extract 5.0
Liver digest 2.5
Sodium chloride 5.0
Agar 12.0
Total 39.5
*Adjusted/supplemented as required to meet performance requirements


Suspend 39.5g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 42-46°C and aseptically add the desired concentration of horse or sheep blood and selective supplements if required. Homogenize gently and dispense into appropriate sterile containers.