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Alkaline Peptone Water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. The high pH of the medium inhibits most enteric organisms for at least 24 hours. The medium is heavily inoculated with faeces and after not more than 8 hours incubation a loopful from the top of the medium is sub cultured onto TCBS Agar. This enrichment medium is also used for food and water testing. -
A very nutritious isotonic general purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
An enriched general purpose broth being an isotonic medium with Tryptose providing a wide range of substrates. The medium is further enriched with 10% horse serum for more fastidious organisms and as an enrichment broth. -
This medium is used to detect and/or confirm the presence of coli-aerogenes group of organisms in water, food and dairy laboratories. Bile and Brilliant Green are included in the medium to inhibit gram positive organisms while the coli-aerogenes organisms are identified by the formation of gas during the fermentation of Lactose. The medium can also be used for the confirmation of Escherichia coli by incubating at 44°C. -
Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms. -
A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002). -
Buffered Sodium Chloride Peptone Diluent is used for the microbial examination of pharmaceutical products, e.g. as a diluent for sample preparation or as a rinsing solution. This formulation complies with the Harmonized USP/EP/JP. -
This is one of a number of selective enrichment broths that can be used in the isolation of Campylobacter spp from clinical, food and environmental specimens and contains nutrients to aid in the resuscitation of damaged organisms. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Vancomycin, Cefoperazone, Trimethoprim and Amphotericin B. Following incubation at 37ºC the broth is usually sub-cultured onto an appropriate solid Campylobacter medium. -
Dey & Engley Broth has the ability to neutralise antimicrobial chemicals and is used for environmental sampling for the detection and enumeration of microorganisms present in cosmetic and/or environmental samples. In this formulation the broth has been modified by the addition of Tween 80 (Polysorbate 80), Lecithin, Sodium thioglycollate, Sodium thiosulphate and Sodium bisulphite. It is recommended for use in environmental testing, particularly in areas subjected to surface disinfection, and conforms to the requirements of the British Pharmacopeia for sterility testing of appropriate pharmaceutical products. The product also conforms to ISO 21149 to act as a diluent in the method for the enumeration of aerobic bacteria from cosmetic samples. Lecithin and Polysorbate 80 (Tween 80) inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). Sodium thioglycollate neutralises mercurials, Sodium thiosulphate neutralises iodine and chlorine and Sodium bisulphite neutralises aldehydes. Bromocresol purple is incorporated into the medium to indicate glucose utilisation. -
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated horse blood is aseptically collected whole horse blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of horse blood are not identical to sheep blood and blood agar media designed for horse blood may not be satisfactory with sheep blood and vice versa. -
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated sheep cells are aseptically collected whole sheep blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of sheep blood are not identical to the reactions of horse blood and blood agar media designed for sheep blood may not be satisfactory with horse blood and vice versa. -
For in vitro diagnostic use. BM0650 Deionised Water is used as a diluent for, where required, the preparation of clinical specimens and microbiological samples, the dilution of microorganisms and reconstitution of lyophilised antibiotic supplements during culture media preparation. Deionised Water is purified water that can be used as a general-purpose diluent in many areas of the laboratory. It may be used for creating 0.5 McFarland suspensions of bacterial isolates for identification tests or processing clinical specimens or industrial samples prior to microbiological examination. -
All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
Legionellae are more resistant to lower pH and brief exposure to higher temperatures than many other freshwater bacteria. For some water samples with high concentrations of bacteria, it is necessary to use a selective procedure to reduce the number of non-Legionella bacteria before culture. Acid treatment and heating of samples are routinely used for this purpose and to aid antigen extraction. The use of this buffer is recommended in the International Standard ISO 11731-2 "Water quality-Detection and enumeration of Legionella" for this purpose. -
E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms. This formulation complies with the Harmonized USP/EP/JP.
