Legionellae are more resistant to lower pH and brief exposure to higher temperatures than many other freshwater bacteria. For some water samples with high concentrations of bacteria, it is necessary to use a selective procedure to reduce the number of non-Legionella bacteria before culture. Acid treatment and heating of samples are routinely used for this purpose and to aid antigen extraction. The use of this buffer is recommended in the International Standard ISO 11731-2 “Water quality-Detection and enumeration of Legionella” for this purpose.
BM1328 – Double Strength Acid Buffer
|Shelf Life (days)|
|Storage Temp (°C)|
|Tested for sterility only by innoculation of Columbia Blood Agar and incubation at 37°C for 36-48 hours|