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This buffer is intended primarily for use as a neutralising agent following treatment with alkaline compounds during the decontamination and homogenisation process of Sputum specimens prior to inoculation onto appropriate culture media for the isolation of Mycobacterium spp. -
A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours. -
Trichomonas Broth (CPLM) with Nystatin is for the selective isolation of Trichomonas spp. This medium is based on the formula described by Kupferburg et al.. The superiority of the culture procedure over the wet mount procedure for detecting the presence of trichomonads in clinical specimens was demonstrated by Williams, and Kean and Day. Feinberg and Whittington demonstrated the greater accuracy of the culture procedure for detecting trichomonads in clinical material. The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate. The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of Trichomonas spp.. Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium. The medium is selective due to the inclusion of chloramphenicol and nystatin to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. Cultures may be examined microscopically after 48 hours incubation at 37 ± 1°C for the presence of flagellate protozoans. If a negative result is obtained, then the culture may be re-incubated for a further 72 hours. -
Tryptone Soya Broth (Modified) with Novobiocin (20mg/L) This is a selective enrichment broth for the isolation of Escherichia coli 0157, primarily from food and food products, and is capable of detecting the organisms even when they are present in small numbers. It is also increasingly being used in clinical laboratories when screening faecal samples. Based on Tryptone Soya Broth it is made selective for Escherichia coli 0157 by the addition of bile salts and Novobiocin and is also buffered to maintain the pH during incubation. This medium is generally used in conjunction with selective agar subculture (e.g. Sorbitol MacConkey Agar with Cefixime Tellurite – (CT-SMAC)). -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). -
A general purpose broth suitable for the cultivation of most micro-organisms including many fastidious organisms and fungi. It is recommended by the United States Pharmacopoeia for sterility testing of many pharmaceutical products. -
This is a general-purpose broth for cultivation of fastidious bacteria, yeasts and moulds. The medium incorporates Polysorbate 80 to act as an emulsifying agent and inactivate phenols. Lecithin is also incorporated to inactivate quaternary ammonium compounds. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, and other areas of bacteriological investigation.
