BM0412 – Trichomonas Broth (CPLM)

This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours.