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A sterile concentrated emulsion of premium egg yolks, suitable for incorporation in culture media which detect lecithinase production by bacteria. It can be used in media for Bacillus cereus and Staphylococci. Used with serum and Filde’s extract it may be used to produce Nagler plates for Clostridia. -
This is a sterile emulsion of Egg Yolk in Saline containing Potassium Tellurite and is generally used as a selective differential agent in Baird Parker Medium. The complete medium is selective for Staphylococcus aureus as the Potassium Tellurite inhibits most coliform organisms and is also reduced by Staphylococcus aureus to tellurite giving typical black colonies on the Baird Parker Medium. -
Enterobacteriaceae Enrichment Broth E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non lactose fermenting organisms. -
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present.
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A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. This formulation complies with the United States Pharmacopoeia. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. Fluid D has been formulated with the addition of polysorbate 80 to enable the rinsing of products containing lecithin or oil. This formulation complies with the Harmonised USP/EP/JP.
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This medium is intended for use in sterility testing of substances in accordance with the United States Pharmacopoeia and European Pharmacopoeia. It is a general purpose medium for the cultivation of both fastidious anaerobic and aerobic micro-organisms. Sodium Thioglycollate and L-cystine are included to reduce the medium to ensure anaerobiosis and to inactivate mercury and other heavy metallic compounds. A small amount of agar is added to further reduce the update of oxygen. However, in the event of oxidation, the medium will turn pink due to the presence of the resazurin. Providing only 30% of the medium has turned pink, the Eh may be restored by heating the medium (once only) in a boiling water bath. -
A modification of UVM Medium, Fraser Broth is a secondary selective enrichment broth for the isolation of Listeria spp primarily from food and environmental specimens. The medium is made selective by the inclusion of Nalidixic Acid and Acriflavine. Darkening of the broth following incubation, due to the presence of Aesculin and Ferric Ammonium Citrate, is indicative of the presence of Listeria spp. Lithium Chloride is also included to inhibit the growth of enterococci that would otherwise hydrolyse the Aesculin. This medium is generally used in conjunction with Fraser Broth Half-Strength (BM0647). NB: It should be noted that the lack of darkening of the broth should not be taken as a final negative result and all Fraser Broth enrichment cultures should be sub-cultured onto an appropriate selective agar medium irrespective of colour. -
A modification of UVM11 Medium, Fraser Broth Half-Strength is a primary selective enrichment broth for the isolation of Listeria spp. from food and environmental samples and is generally used in conjunction with Fraser Broth (BM0640). Although the base is identical to Fraser Broth it differs in that it contains only half the quantity of selective agents (Nalidixic acid and Acriflavine). -
A medium for the selective isolation of group B streptococci. This information has been obtained directly from the HPA files at the customers request. -
Lysed horse blood is used for special purposes in culture media. It has been used for many years in Corynebacterium diphtheriae media, where better growth was observed after lysis of the horse blood by the tellurite in the medium. It is also documented that lysed blood stimulates the growth of Haemophilus influenzae due to the release of Nicotinamide Adenine Dinucleotide in the horse blood from the ruptured erythrocytes. In antibiotic susceptibility testing, lysed horse blood is added to the medium to improve the reactions with trimethoprim and sulphonamides. Most culture media, unless specially processed for susceptibility testing, contain amounts of thymidine which can antagonise the inhibitory effects of these antimicrobials. When horse blood is lysed the erythrocytes release an enzyme thymidine phosphorylase which converts thymidine into the much less antagonistic compound thymine. -
This is a chemical complex which should be used in conjunction with BM0945 Muller – Kauffmann Tetrathionate Broth Base. It is recommended that the complex is used as a 2% solution with the base media and should be only added on the day of use. -
Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing. -
Luria Bertani broth (LB Broth) is a nutrient broth primarily used for the growth and maintenance of Escherichia coli. Used as the primary propagation step for donor or recipient cells, when further work is to be performed on LB Agar. -
A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
