This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 – Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth.
BM0945 – Muller-Kauffmann Tetrahionate Novobiocin (MKTTn) Broth
|Shelf Life (days)|
|Storage Temp (°C)|
When used in the examination of food samples reference should be made to the document 'ISO 6579:2002' for full details of methodolgy. OTHERWISE: aerobically at 37°C ± 1°C for 18-24 hours.
|Escherichia coli||NCTC 12241||Inhibited|
|Pseudomonas aeruginosa||NCTC 12903||Inhibited|
|Enterococcus faecalis||NCTC 12697||Inhibited|
|Salmonella typhimurium||NCTC 12023||Growth|