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BM0370 Selenite Mannitol Broth is a selective enrichment medium used for the isolation of Salmonella species from clinical, food and environmental specimens. Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media. Selenite Mannitol Broth is recommended for the enrichment of Salmonella species from faecal specimens in UK SMI S7 for the investigation of gastroenteritis.
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Maximum Recovery Diluent (Peptone/ Saline Diluent) An osmotically controlled solution for the preparations of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The presence of a low level peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringers Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent.
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For the enumeration of Escherichia coli and coliform organisms in water using a Membrane Filtration Technique. Previously known as Membrane Enriched Teepol Broth, Lauryl Sulphate has replaced Teepol 610, which is no longer available. Phenol Red is included in the medium making it possible for coliforms to be more readily detected following incubation.
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A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. This medium allows for the detection of gas when a Durhum tube is incorporated in the medium.
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A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. Which allows the detection of acid & gas when a Durhum tube is incorporated in the medium.
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MSS is a viral transport solution designed to facilitate collection and transport of viruses safely from site of sampling to a laboratory location at ambient temperatures. The medium is buffered to help maintain a pH of 7.1, and contains a metal ion chelator, a non-ionic surfactant and a chaotropic agent to ensure disruption of virus particles, denaturation of viral proteins and inactivation of nucleases that may destroy target nucleic acids. The resultant sample is suitable for virus identification by PCR analysis as well as other nucleic acid manipulations. MSS is designed to disrupt viral particles, denature viral proteins and, therefore, reduce the infection hazard during transport and laboratory processing. NB – BM1677 MSS has been validated only for use with the SARS-CoV-2 virus 2ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Storage: 2-30°C • Store in the dark • Shelf life: 730 days • Samples collected in MSS are suitable for PCR analysis and other nucleic acid manipulations. • MSS is designed to disrupt viral particles, denature viral proteins, inactivate nucleases and reduce the infection hazard during transport and laboratory processing.
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Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim. It is sometimes used in conjunction with Mueller-Hinton Agar.
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This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth.
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This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth.
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A general-purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements.
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Based on Nutrient Broth with an additional 2.5% Sodium Chloride this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus especially MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media as described in PHE SMI B29 issue No.6.
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Based on Nutrient Broth with an additional 6.0% Sodium Chloride (Total Sodium Chloride content = 6.5%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media.
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Based on Nutrient Broth with an additional 6.5% Sodium Chloride (Total Sodium Chloride content = 7%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media.
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This is an aqueous solution of Oxalic acid (3%) suitable for use in the digestion and neutralisation of Sputum that may be contaminated with Pseudomonas like organisms prior to culture.
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This is an aqueous solution of Oxalic acid (5%) suitable for use in the digestion and neutralisation of Sputum that may be contaminated with Pseudomonas like organisms prior to culture.
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A general-purpose medium that can be used as a base for carbohydrate fermentation media. It has a high level of Tryptone and is therefore also suitable for use in Indole testing.