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A modification of UVM Medium, Fraser Broth is a secondary selective enrichment broth for the isolation of Listeria spp primarily from food and environmental specimens. The medium is made selective by the inclusion of Nalidixic Acid and Acriflavine. Darkening of the broth following incubation, due to the presence of Aesculin and Ferric Ammonium Citrate, is indicative of the presence of Listeria spp. Lithium Chloride is also included to inhibit the growth of enterococci that would otherwise hydrolyse the Aesculin. This medium is generally used in conjunction with Fraser Broth Half-Strength (BM0647). NB: It should be noted that the lack of darkening of the broth should not be taken as a final negative result and all Fraser Broth enrichment cultures should be sub-cultured onto an appropriate selective agar medium irrespective of colour. -
A modification of UVM11 Medium, Fraser Broth Half-Strength is a primary selective enrichment broth for the isolation of Listeria spp. from food and environmental samples and is generally used in conjunction with Fraser Broth (BM0640). Although the base is identical to Fraser Broth it differs in that it contains only half the quantity of selective agents (Nalidixic acid and Acriflavine). -
A medium for the selective isolation of group B streptococci. This information has been obtained directly from the HPA files at the customers request. -
Lysed horse blood is used for special purposes in culture media. It has been used for many years in Corynebacterium diphtheriae media, where better growth was observed after lysis of the horse blood by the tellurite in the medium. It is also documented that lysed blood stimulates the growth of Haemophilus influenzae due to the release of Nicotinamide Adenine Dinucleotide in the horse blood from the ruptured erythrocytes. In antibiotic susceptibility testing, lysed horse blood is added to the medium to improve the reactions with trimethoprim and sulphonamides. Most culture media, unless specially processed for susceptibility testing, contain amounts of thymidine which can antagonise the inhibitory effects of these antimicrobials. When horse blood is lysed the erythrocytes release an enzyme thymidine phosphorylase which converts thymidine into the much less antagonistic compound thymine. -
This is a chemical complex which should be used in conjunction with BM0945 Muller – Kauffmann Tetrathionate Broth Base. It is recommended that the complex is used as a 2% solution with the base media and should be only added on the day of use. -
Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing. -
Luria Bertani broth (LB Broth) is a nutrient broth primarily used for the growth and maintenance of Escherichia coli. Used as the primary propagation step for donor or recipient cells, when further work is to be performed on LB Agar. -
A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
