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BM1322 Sodium Hydroxide (4%) is a reagent used in the digestion and decontamination of sputum samples prior to culture for mycobacteria. Most clinical specimens submitted for acid-fast bacilli isolation are contaminated with more quickly developing commensal microbial flora. Additionally, acid-fast bacilli may be retained in respiratory secretions and not released for culture until the material is liquefied. Respiratory specimens, such as sputum, contain mucin which may trap microorganisms. Decontamination and digestion of the mucous components kills contaminating normal flora and allows slower growing mycobacteria to grow. Timely neutralization prevents potential loss of mycobacteria caused by high pH levels of decontaminants, resulting in the preservation of more viable organisms. BM1322 is recommended by the UK Standards for Microbiology Investigations as part of their decontamination/digestion protocol; it is generally used in conjunction with Sputum Neutralising Buffer (BM1325). -
Sodium Hydroxide (4%) with Phenol Red is a reagent used in the digestion and decontamination of sputum samples prior to culture for mycobacteria. Most clinical specimens submitted for acid-fast bacilli isolation are contaminated with more quickly developing commensal microbial flora. Additionally, acid-fast bacilli may be retained in respiratory secretions and not released for culture until the material is liquefied. Respiratory specimens, such as sputum, contain mucin which may trap microorganisms. Decontamination and digestion of the mucous components kills contaminating normal flora and allows slower growing mycobacteria to grow. Timely neutralization prevents potential loss of mycobacteria caused by high pH levels of decontaminants, resulting in the preservation of more viable organisms. Phenol red is used to indicate changes in pH. BM1320 is recommended by the UK Standards for Microbiology Investigations as part of their decontamination/digestion protocol; it is generally used in conjunction with Sputum Neutralising Buffer without Phenol Red (BM1324). -
This is a sterile aqueous solution of Potassium di-hydrogen phosphate (16.0 % w/v) with Phenol Red Indicator suitable for use in the neutralisation following the digestion of sputum with Sodium hydroxide prior to culture. It is generally used in conjunction with BM1322 Sodium hydroxide 4%. -
This is a sterile aqueous solution of Potassium Di-Hydrogen Ortho-phosphate (16.0 % w/v) suitable for use in the neutralisation following the digestion of Sputum with Sodium Hydroxide prior to culture. It is generally used in conjunction with Sodium Hydroxide 4% with Phenol Red Indicator 0.2%. -
E&O Heat Inactivated Serum is derived from horse serum and is suitable for use in diagnostic assays. One of the reasons for the heat inactivation of serum (heating to 56°C for 30 min) is to inactivate complement, a group of proteins present in serum that are part of the immune response. This is sometimes important for cells that will be used to prepare or assay viruses, used in cytotoxicity assays or other systems where complement may have an unwanted influence. The use of Heat Inactivated Serum is also usually recommended for growing embryonic stem cells. After filtration the dispensing and bottle filling processes are carried out in a state-of-the-art clean room under laminar flow. Once labelled the filled bottles are then subjected to controlled heat inactivation and are frozen and stored at -20°C without delay. The filter sterile Heat Inactivated Horse Serum is supplied in 100 or 500 ml PETG bottles. All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
This product may be used as an alternative pre-treatment solution to lessen the background flora of samples before the testing for Mycobacteria species. This reagent should only be used for samples that routinely produce contaminated cultures after processing with an alkaline digestant. -
This product may be used as a pre treatment solution to lessen the background commensal flora of samples before the testing for Mycobacteria species.
