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An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
An enriched general purpose broth being an isotonic medium with Tryptose providing a wide range of substrates. The medium is further enriched with 10% horse serum for more fastidious organisms and as an enrichment broth. -
This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications. -
Prepared from Minced Meat granules overlaid with Fastidious Anaerobe Broth this medium is suitable for the recovery, isolation and storage of the most fastidious anaerobes and is possibly one of the best variations of Cooked Meat Medium available. Fastidious Anaerobe Broth is designed for optimum growth of all anaerobes, with the growth factors Vitamin K, Haemin and L-Cysteine included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate is also present to reduce the ph of the medium and a small amount of Agar to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. If immediately before use a narrow band of reddish/purple is apparent at the surface of the broth this does not indicate that the medium is unsuitable for use. This is due to the action of oxygen on the redox indicator and the medium should be placed in a boiling water bath, with the cap loosened, for about 15 minutes to remove dissolved oxygen. Immediately on removal from the water bath the cap should be tightened and the medium allowed to cool without agitation. -
Prepared from Minced Meat granules overlaid with Brain Heart Infusion Broth this medium is suitable for recovery, isolation and storage of the most fastidious anaerobes as well as many aerobes. -
This is a defined medium primarily for the cultivation of fungi and bacteria that are capable of utilising Sodium Nitrate as their sole source of Nitrogen. If made more acidic by adjusting to pH 3.5 it can also be used for isolation of yeasts. It has also been used for the identification of Candida albicans by chlamydospore production. -
Enterobacteriaceae Enrichment Broth E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non lactose fermenting organisms. -
A modification of UVM Medium, Fraser Broth is a secondary selective enrichment broth for the isolation of Listeria spp primarily from food and environmental specimens. The medium is made selective by the inclusion of Nalidixic Acid and Acriflavine. Darkening of the broth following incubation, due to the presence of Aesculin and Ferric Ammonium Citrate, is indicative of the presence of Listeria spp. Lithium Chloride is also included to inhibit the growth of enterococci that would otherwise hydrolyse the Aesculin. This medium is generally used in conjunction with Fraser Broth Half-Strength (BM0647). NB: It should be noted that the lack of darkening of the broth should not be taken as a final negative result and all Fraser Broth enrichment cultures should be sub-cultured onto an appropriate selective agar medium irrespective of colour. -
This is a modification of the traditional liquid media used to differentiate and identify micro-organisms. It consists of a Buffered Tryptose base containing Lactose and solidified with Gelatin which permits detection of Gelatin Liquefaction where appropriate. Phenol Red Indicator is also included as an indicator of pH change. -
This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. -
This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth. -
Based on Nutrient Broth with an additional 2.5% Sodium Chloride this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus especially MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media as described in PHE SMI B29 issue No.6. -
Based on Nutrient Broth with an additional 6.0% Sodium Chloride (Total Sodium Chloride content = 6.5%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
Based on Nutrient Broth with an additional 6.5% Sodium Chloride (Total Sodium Chloride content = 7%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution with added Tween is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium. It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
A long established selective medium for the isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium inhibits most bacteria and spore structures and pigmentation of fungi are generally well developed on this medium. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp particularly Mycobacterium bovis. This medium is used primarily in the veterinarian sector. The medium is based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
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This is a plate count agar originally suggested by the American Public Health Association for the estimation of total viable counts in food and dairy products.
