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This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. NB: this media will NOT isolate M.bovis which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. This media will isolate most common mycobacteria including M.bovis. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium. -
BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is designed for use with sodium hydroxide (NaOH) treated specimens that have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto the LJ medium after concentration by centrifugation. This medium will isolate most common mycobacteria including M. tuberculosis and can be used for the presumptive identification of mycobacterial infections, testing antibiotic susceptibility of isolates, and differentiating different species of Mycobacterium (by colony morphology, growth rate, biochemical characteristics, and microscopy). BM8200 is recommended by the UK Standards for Microbiology Investigations. NB: This media will not isolate wild strains of M. bovis, which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope such as E&O BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium. -
BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium bovis. BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium is designed for use with sodium hydroxide (NaOH) treated samples which have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto LJ media after concentration by centrifugation. This medium differs from E&O BM8200 Non-acidified Glycerol Lowenstein Jensen Medium in that sodium pyruvate has replaced the glycerol, which has been demonstrated to be inhibitory to some species, particularly M. bovis. BM8300 is recommended by the UK Standards for Microbiology Investigations and tested in accordance with the principals of ISO 11133:2014. The medium contains an egg emulsion which provides the required protein and fatty acids. Sodium pyruvate is included as an alternate to glycerol to allow favourable growth of M. bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green and penicillin G are incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 36 ± 1°C for 8 weeks, checking weekly for growth. Further incubation up to 12 weeks may be required for certain Mycobacterium species. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp particularly Mycobacterium bovis. This medium is used primarily in the veterinarian sector. The medium is based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium.
