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An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
An enriched general purpose broth being an isotonic medium with Tryptose providing a wide range of substrates. The medium is further enriched with 10% horse serum for more fastidious organisms and as an enrichment broth. -
This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications. -
Prepared from Minced Meat granules overlaid with Fastidious Anaerobe Broth this medium is suitable for the recovery, isolation and storage of the most fastidious anaerobes and is possibly one of the best variations of Cooked Meat Medium available. Fastidious Anaerobe Broth is designed for optimum growth of all anaerobes, with the growth factors Vitamin K, Haemin and L-Cysteine included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate is also present to reduce the ph of the medium and a small amount of Agar to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. If immediately before use a narrow band of reddish/purple is apparent at the surface of the broth this does not indicate that the medium is unsuitable for use. This is due to the action of oxygen on the redox indicator and the medium should be placed in a boiling water bath, with the cap loosened, for about 15 minutes to remove dissolved oxygen. Immediately on removal from the water bath the cap should be tightened and the medium allowed to cool without agitation. -
Prepared from Minced Meat granules overlaid with Brain Heart Infusion Broth this medium is suitable for recovery, isolation and storage of the most fastidious anaerobes as well as many aerobes. -
This is a defined medium primarily for the cultivation of fungi and bacteria that are capable of utilising Sodium Nitrate as their sole source of Nitrogen. If made more acidic by adjusting to pH 3.5 it can also be used for isolation of yeasts. It has also been used for the identification of Candida albicans by chlamydospore production. -
Enterobacteriaceae Enrichment Broth E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non lactose fermenting organisms. -
This is a modification of the traditional liquid media used to differentiate and identify micro-organisms. It consists of a Buffered Tryptose base containing Lactose and solidified with Gelatin which permits detection of Gelatin Liquefaction where appropriate. Phenol Red Indicator is also included as an indicator of pH change. -
This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. -
This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth. -
Based on Nutrient Broth with an additional 2.5% Sodium Chloride this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus especially MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media as described in PHE SMI B29 issue No.6. -
Based on Nutrient Broth with an additional 6.0% Sodium Chloride (Total Sodium Chloride content = 6.5%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
Based on Nutrient Broth with an additional 6.5% Sodium Chloride (Total Sodium Chloride content = 7%) this medium is suitable for use in the investigation of outbreaks involving Staphylococcus aureus including MRSA. The additional Sodium Chloride inhibits most other organisms allowing the staphylococci to multiply freely even if present in small numbers. The medium is generally used as an enrichment medium in conjunction with subculture onto selective solid media. -
Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution with added Tween is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
This medium is recommended for the detection and enumeration of yeasts and moulds in food and dairy products. It can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. The low pH (5.6) suppresses the growth of most bacteria and the low mineral content ensures good pigment production by fungi where appropriate. -
This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium. It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
A long established selective medium for the isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium inhibits most bacteria and spore structures and pigmentation of fungi are generally well developed on this medium. -
A medium for the selective enrichment of Salmonellae spp from both clinical and food samples. It is a buffered Lactose Peptone Broth to which Sodium Biselenite is added as the selective agent and Cystine to enhance the recovery of salmonella in low numbers. Subcultures should be made from the top 1/3 of the broth after not more than 24 hours incubation as after this time there is a loss of selectivity.
