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This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours. -
Trichomonas Broth (CPLM) with Nystatin is for the selective isolation of Trichomonas spp. This medium is based on the formula described by Kupferburg et al.. The superiority of the culture procedure over the wet mount procedure for detecting the presence of trichomonads in clinical specimens was demonstrated by Williams, and Kean and Day. Feinberg and Whittington demonstrated the greater accuracy of the culture procedure for detecting trichomonads in clinical material. The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate. The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of Trichomonas spp.. Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium. The medium is selective due to the inclusion of chloramphenicol and nystatin to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. Cultures may be examined microscopically after 48 hours incubation at 37 ± 1°C for the presence of flagellate protozoans. If a negative result is obtained, then the culture may be re-incubated for a further 72 hours.
