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Brazier's CCEY Agar with 1% Lysed Horse Blood - Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light. -
Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms. -
A pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective medium. This non-selective, nutritious medium is free from inhibitors and is well buffered to maintain the pH at 7.0 for the incubation period according to ISO 6579 (2002). -
Buffered Sodium Chloride Peptone Diluent is used for the microbial examination of pharmaceutical products, e.g. as a diluent for sample preparation or as a rinsing solution. This formulation complies with the Harmonized USP/EP/JP. -
Colorex™Acinetobacter MDR is a chromogenic medium for the detection of multi-drug resistant (MDR) Acinetobacter spp. Positive colonies exhibit a distinct red colouration with a pale grey centre enabling easy interpretation amongst blue, violet or colourless colonies that may be produced by other Gram –ve bacteria. Gram +ve bacteria and yeast are inhibited on this medium. Limitations: 1. Some Pseudomonas, Stenotrophomonas and Burkholderia spp. may form pale red colonies on this medium but are readily distinguishable due to differences in colonial morphology compared to the Acinetobacter spp. An oxidase test will readily differentiate any Pseudomonas spp. 2. Some Enterobacteriaceae isolates may form blue colonies on this medium. 3. Definitive MDR characterisation may require additional antibiotic susceptibility testing. -
Colorex C3GR is a chromogenic screening medium for the detection of β-Lactamase producing Gram-negative bacteria in clinical specimens. The selectivity of the medium allows for detection of ESBL and/or AmpC producing isolates that exhibit a reduced susceptibility to 3rd generation cephalosporin antibiotics. The chromogenic reactions allow for species differentiation on presumptive positive isolates. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the Enterobacteriaceae. Colorex™ ESBL (Extended Spectrum Beta-Lactamase) medium has been developed for the isolation of ESBL – producing organisms with the aim of simplifying the differentiation and presumptive identification of the causative organism. It should be noted that other non-ESBL and AmpC isolates will be inhibited on this medium reducing the incidence of false positives. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium. Typical colour reactions are as follows: Escherichia coli – Red colonies; Proteus spp., Providencia spp. & Morganella spp. – Clear colonies with a brown halo; Klebsiella spp., Enterobacter spp, Serratia spp. & Citrobacter spp. – Metallic blue colonies; Salmonella spp. & Acinetobacter spp. – Clear colonies; Gram +ve bacterial species and yeasts – Inhibited. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. Colorex™ UTI has been developed primarily for use in the examination of urine specimens with the aim of simplifying the differentiation and presumptive identification of the main organisms, gram negative and gram positive, usually found in Urinary Tract Infections. It can however be used to differentiate organisms in other types of clinical specimens. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium. -
Glucose agar allows for the detection of glucose fermentation (with or without gas production) as an identification test for Enterobacteriaceae (ISO 21528) and B.cereus (ISO 7932). It is further enriched by the addition of Yeast Extract. Bromocresol Purple is also included and acts as an indicator of glucose fermentation. -
This is a selective medium for the isolation and enumeration of enterococci (Group D streptococci) primarily in food although it has found uses in other areas of bacteriology. The medium is made selective by the inclusion of Kanamycin and Sodium Azide while Aesculin and Ferric Ammonium Citrate act as the indicator system. -
Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This media has the addition of Kanamycin at 0.05gms/L for use with Kanamycin resistant strains and cells harbouring selection plasmids containing the Kanamycin resistance gene.Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. -
Luria Bertani broth (LB Broth) is a nutrient broth primarily used for the growth and maintenance of Escherichia coli. Used as the primary propagation step for donor or recipient cells, when further work is to be performed on LB Agar. -
Letheen Broth with Neutraliser and 1% Tween 80 is primarily intended for use in assessing the bactericidal activity of quaternary ammonium compounds and determining the phenol coefficient of cationic surfactants. It can also be used in environmental testing, particularly in areas subjected to surface disinfection. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol). -
Maximum Recovery Diluent (Peptone/ Saline Diluent) An osmotically controlled solution for the preparations of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The presence of a low level peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringers Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent. -
This is a general-purpose neutralising diluent used particularly in the pharmaceutical industry. Lecithin, L-histidine and Tween 80 are present to inactivate surface disinfectants such as quaternary ammonium compounds, phenols, aldehydes (including formaldehyde), hexachlorophene and ethanol. The diluent may be used in sampling surfaces and equipment (including endoscopes) to detect the presence of surviving microorganisms after disinfection. The presence of the surfactant Tween 80 also helps release adherent organisms from surfaces being tested.
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This is an established medium, with a neutral pH, used for the enumeration of Yeasts and Moulds -
A general-purpose diluent for the preparation of test samples according to ISO/DIS 6887-5. -
Plate Count Agar (APHA) (Standard Methods Agar, Tryptone Glucose Yeast Agar) This medium is formulated to A.P.H.A. specification and intended for use in food, dairy and water bacteriology to perform Total Viable Counts. The agar is of high gel strength and is therefore suitable for use in pour plate techniques as well as surface inoculation. -
Originally intended for use in surface counting and pour plating techniques this medium can be used as a general purpose medium for the cultivation of most organisms particularly those that are less fastidious in their nutritional requirements. Can also be used as a maintenance medium for stock cultures. -
RPMI Medium for E-Test RPMI Medium is recommended for use in anti-fungal susceptibility testing of yeasts from clinical isolates using the E-Test method. The medium is based on a simple Glucose Agar with added RPMI-1640 Medium (without Sodium Bicarbonate & Phenol Red), which supplies the necessary vitamins and amino-acids, and MOPS (3-(Morpholino)propanesulfonic Acid) Buffer to maintain the medium pH during incubation. -
BM0397 Saline Tryptone Water (pH 7.0) is isotonic saline containing 0.1% tryptone that may be used as a general-purpose diluent in many areas of the laboratory. It is recommended by The British Standards Institute (1) and is tested according to the principles of ISO 11133:2014 (2). The product does not interfere with biochemical reactions used to identify organisms, such as indole production.
References
- The British Standards Institution (2015) BS EN 16615:2015 Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal activity on non-porous surfaces with mechanical action employing wipes in the medical area (4- field test). Test method and requirements (phase 2, step 2). Published by BSI Standards Limited.
- International Organization for Standardization (2014) 11133:2014 Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media. Geneva, ISO.
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This medium can be used as a screening method for the differentiation of Enterobacteriaceae based on the ability of some species to utilise citrate as a sole source of carbon. It is often used as a screening test for Klebsiella pneumoniae (Positive reaction) while Escherichia coli is negative. Other uses included distinguishing between species of citrate positive Salmonellae (e.g. Salmonella enteritidis) and those that are negative (e.g. Salmonella typhi and Salmonella paratyphi A). The medium is inoculated by stabbing the organism (in pure culture) into the medium. A positive result produces a change of colour from green to bright blue and a negative reaction leaves the colour unchanged. -
This is a plate count agar originally suggested by the American public Health Association for the estimation of total viable counts in food and dairy products. -
This is a plate count agar originally suggested by the American Public Health Association for the estimation of total viable counts in food and dairy products.
