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A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of bromocresol purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of ox bile helps to suppress the growth of Gram-positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP. Nitrogen is supplied by the gelatin peptone whilst lactose serves as the fermentable carbohydrate source. Oxbile is the selective agent helping to suppress Gram-positive organisms and bromocresol purple detects the pH change as a result of the fermentation of lactose. -
Originally intended for enumeration of coliforms in water samples, a medium containing glutamic acid was devised by Folpmers and further developed by Gray as a potential replacement for MacConkey Broth. Later, Gray modified the medium to incorporate a variety of minerals promoting improved lactose fermentation by coliforms and Escherichia coli. Minerals Modified Glutamate Medium is suitable for the enrichment of low levels of coliforms and recovery of chlorine-damaged bacteria.
Minerals Modified Glutamate Medium is recommended by the Standing Committee of Analysts and International Organization for Standardization. It is tested in accordance with ISO 11133:2014.
