KM0125 – MacConkey Broth (USP)

//KM0125 – MacConkey Broth (USP)

KM0125 – MacConkey Broth (USP)

A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of bromocresol purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of ox bile helps to suppress the growth of Gram-positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.

Nitrogen is supplied by the gelatin peptone whilst lactose serves as the fermentable carbohydrate source. Oxbile is the selective agent helping to suppress Gram-positive organisms and bromocresol purple detects the pH change as a result of the fermentation of lactose.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Escherichia coliATTC 8739Growth, acid & gas production
Staphylococcus aureusATTC 6538Inhibited

Formula*

FORMULA*g/L
Pancreatic digest of gelatin20
Lactose10
Oxbile5
Bromocresol purple0.01
Total35

Preparation

Suspend 35.0 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.

Dispense into suitable containers with Durham tubes added. Sterilise at 121°C for 15 minutes.