Middlebrooks 7H11 Selective Agar (No Glycerol)

///Middlebrooks 7H11 Selective Agar (No Glycerol)

Middlebrooks 7H11 Selective Agar (No Glycerol)

This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis.

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Product Description

Organisms Ref. No Result
Mycobacterium tuberculosis ATCC 35818 Growth
Mycobacterium bovis NCTC 5692 Growth
Mycobacterium kansasii NCTC 13024 Growth
Mycobacterium fortuitum NCTC 10394 Growth
Mycobacterium intracellulare NCTC 10682 Growth



FORMULA   gm/litre
Pancreatic digest of casein 1.0
L-Glutamic acid 0.5
Sodium pyruvate 4.0
Sodium citrate 0.4
Pyridoxine 0.001
Biotin 0.0005
Ferric ammonium citrate 0.04
Ammonium sulphate 0.5
Di-sodium phosphate 1.5
Mono-potassium phosphate 1.5
Magnesium sulphate 0.05
Agar 13.5
Calcium chloride 0.0005
Malachite green 0.00025
Zinc sulphate 0.001
Copper sulphate 0.001
Middlebrook OADC Supplement 100mls
Lysed sheep blood 5mls
Adult bovine serum 100mls
Amphotericin B 0.01
Ticarcillin 0.1
Trimethoprim 0.01
Polymixin B 200,000IU