This medium is designed for the selective isolation of Campylobacter spp., particularly C. jejuni and C. coli , which are major causes of gastrointestinal infection. Campylobacter Blood Free Agar CCDA was formulated to allow replacement of blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate. Selectivity is achieved through the addition of broad spectrum cefoperazone and amphotericin B to inhibit fungi. Inoculated plates are incubated at 41.5°C to improve growth of the thermophilic species including C. jejuni and C. coli. Campylobacter Blood Free Agar CCDA is recommended for food testing in compliance with the requirements of ISO 10272- 1:2017(1) . It can also be used for isolation of Campylobacter spp. from clincal specimens such as faeces(2,3).
1. ISO 10272-1:2017. Microbiology of food and animal feeding stuffs – Horizontal method for detection and enumeration of Campylobacter spp. – Part 1: Detection method.
2. Public Health England. (2014). Investigations of Faecal Specimens for Enteric Pathogens. UK Standards for Microbiology Investigations. B 30 Issue 8.1.
3. Public Health England. (2018). Identification of Campylobacter species. UK Standards for Microbiology Investigations. ID 23 Issue 3.1.