TCBS Cholera Medium

Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar
TCBS is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical samples. The formulation was developed by Kobayashi, Enomoto, Skazaki and Kuwahara. This medium inhibits most enterobacteriacae for at least 24 hours. For the isolation of Vibrio spp. other than V.cholerae in environmental bacteriology, it is advisable to incubate at the lower temperature range of 20°C – 30°C.

NB – It is not recommended to perform an oxidase test on any presumptive positive isolates directly from TCBS medium.

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Organisms Ref. No Result
Vibrio furnisii NCTC 11218 Yellow colonies
Vibrio cholerae NCTC 11348 Yellow colonies
Escherichia coli NCTC 12241 Inhibited
Vibrio parahaemolyticus NCTC 10885 Green colonies