Side 1 – Columbia Agar w 5% Horse Blood
This is a general-purpose medium with 5% defibrinated horse blood suitable for isolation of most organisms including fastidious anaerobes.
Side 2- Chocolate Agar w 5% Horse Blood
This is a highly nutritious medium supplemented with defibrinated horse blood and chocolated by heating to 70°C for 5 minutes. It will support the growth of a wide range of pathogens including the most fastidious organisms and is particularly useful for the cultivation of Haemophilus spp. and Neisseria spp
Columbia Agar with 5% Horse Blood / Columbia Chocolate Agar with 5% Horse Blood Biplate
Additional Information
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Product Description
Side 1 – Columbia Agar with 5% Horse Blood
| Organisms | Ref. No | Result |
| Escherichia coli | WDCM 00013 / NCTC 12241 | Grey colonies |
| Streptococcus pyogenes | NCTC 12696 | White colonies with β-haemolysis |
| Streptococcus pneumoniae | NCTC 12977 | Grey/green colonies with α-haemolysis |
| Staphylococcus aureus | NCTC 12981 | White / pale yellow colonies with β-haemolysis |
Recommended incubation: Incubate E. coli, S. aureus and S. pyogenes aerobically at 37 ± 1°C for 18-24 hours.
Incubate S. pneumoniae at 37 ± 1°C in 5% CO2 for 18-24 hours
Side 2 – Chocolate Agar with 5% Horse Blood
| Organisms | Ref. No | Result |
| Haemophilus influenzae | NCTC 12699 | Grey colonies |
| Neisseria gonorrhoeae | NCTC 8375 | Grey colonies |
| Streptococcus pneumoniae | NCTC 12977 | Green colonies with α-haemolysis |
Recommended Incubation: Incubate at 37 ± 1°C in 5-10% CO2 for 24-48 hours.
