KM0076 – Burkholderia Cepacia Agar Base

//KM0076 – Burkholderia Cepacia Agar Base

KM0076 – Burkholderia Cepacia Agar Base

Burkholderia cepacia agar base is a selective medium for the detection and isolation of Burkholderia cepacia from cystic fibrosis (CF) patients. This is an important opportunistic pathogen in CF patients and can lead to fatal infection in approximately 20% individuals that have been colonised with B. cepacia complex organisms. This medium is based on the PC medium described by Gilligan et al.
Magnesium sulphate, ammonium sulphate and ferrous ammonium sulphate supports the growth of B. cepacia. Potassium di-hydrogen phosphate and di-sodium hydrogen phosphate are buffering agents, used to maintain the pH the medium. The phenol red is used as a pH indicator. If the sodium pyruvate in the medium is metabolised by B. cepacia alkaline by-products are produced which raises the pH. This causes the colour of the medium to turn pink/red around sections of heavy growth on the medium. Bile salts and crystal violet are selective agents.
The associated selective supplement for this medium, LS0125, contains ticarcillin and polymyxin B which further improves the selectivity, particularly with the inhibition of Pseudomonas spp.

Related Supplements :
LS0125 B.cepacia Selective Supplement, LS0026 Pseudomonas CFC Selective Supplement

SKU: KM0076 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Burkholderia cepacia NCTC 10661 Growth, grey / green colonies
Escherichia coli NCTC 12241 Inhibited
Pseudomonas aeruginosa NCTC 12903 Inhibited
Staphylococcus aureus NCTC 12981 Inhibited

 

Formula*

g/L
Magnesium sulphate 0.2
Ferrous ammonium sulphate 0.01
Potassium di-hydrogen phosphate 4.35
Di-sodium hydrogen phosphate 1.42
Bile salts 0.5
Ammonium sulphate 1.0
Peptone mixture 8.9
Crystal violet 0.001
Phenol red 0.02
Sodium pyruvate 5.0
Agar 12.0
Total 33.4
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 33.4g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically add 2 vials of selective supplement LS0125. Mix well and dispense into appropriate sterile containers.