Triple Sugar Iron Agar

//Triple Sugar Iron Agar

Triple Sugar Iron Agar

SKU: KM0168 Category:

Triple sugar iron agar is used to differentiate between some of the enterobacteriacae on the basis of four reactions: fermentation of lactose, glucose and sucrose and the production of hydrogen sulphide.
Beef extract, yeast extract and peptone provide the required nitrogen, carbon and vitamins. Lactose, sucrose and glucose are carbohydrates. Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains osmotic balance.


Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Slant:Acid. Butt: Acid. Gas: +. H2S: -.
Salmonella typhimurium NCTC 12023 Slant: Alkaline. Butt: Acid. Gas: +. H2S: +.
Pseudomonas aeruginosa NCTC 12903 Slant: Alkaline. Butt:alkaline. Gas: -. H2S: -.
Shigella flexneri ATCC 12022 Slant: Alkaline. Butt: Acid. Gas: -. H2S: -.
Proteus mirabilis NCTC 10975 Slant: Alkaline. Butt: Acid. Gas: +. H2S: +.



Beef extract 3.0
Yeast extract 3.0
Peptone 20.0
Sodium chloride 5.0
Lactose 10.0
Sucrose 10.0
Glucose 1.0
Ferric citrate 0.3
Sodium thiosulphate 0.3
Phenol red 0.025
Agar 12.0
Total 65.0
*Adjusted/supplement as required to meet performance requirements


Suspend 65g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.