KM0188 – Fastidious Anaerobe Broth

//KM0188 – Fastidious Anaerobe Broth

KM0188 – Fastidious Anaerobe Broth

This medium is for the growth of fastidious anaerobes, particularly Bacteroides spp. Fastidious anaerobe broth is also suitable for anaerobic blood culture.
The peptone and yeast extract provides the required carbon, nitrogen and vitamins. Haemin, vitamin k and L-cysteine HCl are growth factors required by some anaerobes. Sodium thioglycollate and L-cysteine HCl reduce the Eh of the medium and the agar helps maintain the Eh. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance.
NB: For best results it is recommended that the medium be heated in a boiling water bath, with the cap loosened, and then allowed to cool, with the cap tightened, immediately before use. The cap must be replaced on the container immediately after inoculation.

 

SKU: KM0188 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Bacteroides fragilis NCTC 9343 Growth

 

Formula*

g/L
Peptone 15.0
Yeast extract 10.0
Sodium thioglycollate 0.5
Sodium chloride 2.5
L-cysteine HCI 0.5
Resazurin 0.001
Sodium bicarbonate 0.4
Haemin 0.005
Vitamin K 0.0005
Agar 0.75
Total 29.7
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 29.7 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers then sterilise at 121°C for 15 minutes.