LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This medium has the addition of carbenicillin at 100mg/L for use with transformed cells harbouring selection plasmids containing carbenicillin resistance genes. Peptides and essential amino acids are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract and sodium chloride provides osmotic balance.
LB Agar (Lennox) with Carbenicillin (100mg/L) and 1% Glucose
|Shelf Life (days)
|Storage Temp (°C)
|Escherichia coli (ESBL)
Recommended incubation: Incubate aerobically at 37 ± 1°C for 18-24 hours