LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This medium has the addition of kanamycin at 50mg/L and chloramphenicol at 34mg/L, for use with kanamycin and chloramphenicol resistant strains and cells harbouring selection plasmids containing the kanamycin and rifampicin resistance genes. Peptides, peptones and essential amino acids are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract and sodium chloride provides osmotic balance.
LB Agar (Miller) with Kanamycin (50mg/L) & Chloramphenicol (34mg/L)
|Shelf Life (days)
|Storage Temp (°C)
Recommended incubation: Incubate aerobically at 37 ± 1°C for 18-24 hours