Each 500ml vial contains:
To reconstitute the contents of each vial, aseptically add 2ml of Sterile Distilled Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and add the contents of the vial to 1 Lt of sterile Sorbitol MacConkey Agar Base prepared according to the manufacturer’s instructions and allowed to cool to 45°- 50°C. Mix gently but thoroughly and pour aseptically into sterile Petri Dishes.
Overnight storage at 2°- 8°C is recommended to allow equilibration of the supplement within the medium.