LS0210 – Primary Listeria Selective Supplement

//LS0210 – Primary Listeria Selective Supplement

LS0210 – Primary Listeria Selective Supplement

Primary Listeria Selective Supplement
E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.

 

SKU: LS0210 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Dehydrated Medium Colour

Solubility Description

Product Description

Organisms Ref. No Result
Listeria monocytogenes NCTC 11994 Growth
Listeria innocua NCTC 11288 Growth
Enterococcus faecalis NCTC 12697 Inhibited
Saccharomyces cerevisiae NCPF 3178 Inhibited
Escherichia coli NCTC 12241 Inhibited

 

Formula

Vial Contents Per Vial Per Litre
Ceftazidime 10.0mg 20mg
Amphotericin B 5.0mg 10mg
Polymixin B 5.0mg 10mg
Nalidixic acid 10.0mg 20mg

Procedure

To reconstitute the contents of each vial, aseptically add 2ml of Sterile Deionised Water. Close the vial and gently agitate to assist reconstitution. Avoid frothing and the resulting solution should be a, yellow colour and free from particulate matter. Immediately add the contents of the vial to 500ml of sterile Primary Listeria Selective agar prepared according to the manufacturer’s instructions and allowed to cool to 45- 50°C.
Mix gently but thoroughly and pour aseptically into sterile Petri dishes.