Oxytetracycline (100mgs/L) Selective Supplement
E&O Laboratories Ltd Oxytetracycline Selective Supplement can be used with a glucose, yeast or maltose extract based agar for selective isolation of Yeasts and Moulds from a variety of sample types. Due to the near neutral pH of the medium, Oxytetracycline is added to reduce the risk of bacterial growth when processing materials that may be more heavily contaminated.
Media using oxytetracycline as the selective agent is based on the formulation developed by Mossel et al. who stated that the use of this antibiotic in a medium with a neutral pH gave increased counts of yeasts and moulds from a variety of foodstuffs compared with media which relied on a low pH to suppress bacterial growth.
Oxytetracycline
Additional Information
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Dehydrated Medium Colour | |
| Solubility Description | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
| Saccharomyces | NCPF 3178 | Growth |
| Candida albicans | NCPF 3179 | Growth |
| Escherichia coli | NCTC 12241 | Inhibited |
| Bacillus subtilis | NCTC 10400 | Inhibited |
Formula
Each vial contains Oxytetracycline at a concentration of 0.1gms/Litre
Procedure
To reconstitute each vial aseptically add approximately 2-3ml of Sterile Distilled Water. Close the vial and gently agitate to assist reconstitution.
Add the contents of the vial to the required sterile base medium of choice prepared according to the manufactures instructions and allowed to cool to below 50°C. Mix gently but thoroughly and pour or aseptically dispense into the desired sterile containers or dishes.