LS5004 – Ferric Ammonium Citrate

//LS5004 – Ferric Ammonium Citrate

LS5004 – Ferric Ammonium Citrate

Ferric Ammonium Citrate (FAC) Supplement
E&O Laboratories Ltd Ferric Ammonium Citrate (LS5004) is a
supplement used in the isolation of Listeria spp. with Fraser Broth. Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium.
The medium is intended for the isolation of Listeria spp. from food and environmental samples when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. It is generally accepted that the USDA-FSIS two stage enrichment
method employing UVM primary and secondary enrichment broths is the most suitable for the examination of meat products.

SKU: LS5004 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Dehydrated Medium Colour

PH

Solubility Description

Product Description

Organisms Ref. No Result
Listeria monocytogenes NCTC 11994 Growth & Black colonies

 

Formula

Vial Contents per Vial per Litre
Ferric ammonium citrate 0.25g 0.5g

Procedure

Suspend the recommended manufacturer’s amount of dehydrated media in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 45-50°C, and aseptically add the contents of one vial of Ferric Ammonium Citrate (LS5004).To reconstitute vial add 2 mls of distilled water and mix before addition to the supplemented
dehydrated media.