Middlebrook 7H9 Broth with OADC (40ml/L)

Middlebrook 7H9 Broth with OADC (40ml/L)

This is a liquid medium for growing pure cultures of Mycobacterium spp., including M. tuberculosis, for use in antimicrobial assays and biochemical tests.   The medium is complex but includes L-Glutamic acid, ammonium sulphate, sodium citrate, pyridoxine and biotin as growth factors as well as magnesium sulphate and ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium.

The medium is further enriched by the addition of the Middlebrook OADC Enrichment supplement. OADC contains oleic acid to provide fatty acids for growth promotion, bovine albumin and catalase as protective compounds as well as sodium chloride and dextrose.

Additional Information



Shelf Life (days)

Storage Temp (°C)





Product Description

Organisms Ref. No Result
Mycobacteria tuberculosis ATCC 35818 Growth
Mycobacteria kansasii NCTC 13024 Growth
Mycobacteria fortuitum NCTC 10394 Growth
Mycobacteria intracellulare NCTC 10682 Growth

Recommended incubation: Incubate at 37 ± 1°C in 5% CO2 for up to 8 weeks.