Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. (1&2)
Beef extract and acid hydrolysate of casein provides the required nitrogen, carbon and vitamins in the medium.
Starch absorbs any toxic metabolites produced.
(1) Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. CLSI, Wayne, PA.
(2) MacFaddin, J. 1985. Media for isolation cultivation, identification maintenance of medical bacteria. Williams & Williams, Baltimore.