Mueller Hinton Broth

//Mueller Hinton Broth

Mueller Hinton Broth

SKU: KM0138 Category:

Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. (1&2)
Beef extract and acid hydrolysate of casein provides the required nitrogen, carbon and vitamins in the medium.
Starch absorbs any toxic metabolites produced.

(1) Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. CLSI, Wayne, PA.
(2) MacFaddin, J. 1985. Media for isolation cultivation, identification maintenance of medical bacteria. Williams & Williams, Baltimore.

Additional Information

Shelf Life (days)


Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

Organisms Ref. No Result
Staphylococcus aureus ATCC 25923 Growth
Escherichia coli ATCC 10418 Growth


Recommended incubation: Aerobically at 37°C ± 1°C for 18-24 hours.


Beef extract 2.0
Acid hydrolysate of casein 17.5
Starch 1.5
Total 21.0


*Adjusted/supplemented as required to meet performance requirements


Suspend 21.0g of the medium in one litre of deionised / purified water.
Allow the medium to soak whilst mixing for 10 minutes.
Dispense into appropriate containers and sterilise at 121°C for 10 minutes.