PP0400 – DNase Agar

PP0400 – DNase Agar

DN’ase Medium
DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other Staphylococci based on deoxyribonuclease activity. Following incubation of the plate and confirmation of a pure growth of Staphylococci, the surface of the medium is covered with a small quantity of 1M Hydrochloric Acid to precipitate the DNA. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a clear area around the colonies, described as being DNase positive, whereas coagulase negative Staphylococci do not produce clearing.

NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out.

Additional Information

Container

Pack Size

Shelf Life (days)

Storage Temp (°C)

Appearance

Colour

PH

Fill Volume

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Clearing around colonies *
Staphylococcus epidermidis NCTC 13360 No clearing around colonies*