DNase Agar & Methyl Green

DNase Agar & Methyl Green

DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other staphylococci based on deoxyribonuclease activity. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a colourless zone around the colonies, described as being DNase positive, whereas coagulase negative staphylococci do not produce clearing. This particular formulation can also be used for Streptococci and Serratia.

NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out.

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Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Clearing around colonies
Staphylococcus epidermidis NCTC 13360 No clearing around colonies