Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction).
PP0670 – Nagler Medium
|Shelf Life (days)|
|Storage Temp (°C)|
|Clostridium perfringens||NCTC 8237||Growth with opalescent zone (+ve reaction)|
|Escherichia coli||NCTC 12241||Growth with no zone (-ve reaction)|