Nagler Medium

Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction).

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Product Description

Organisms Ref. No Result
Clostridium perfringens NCTC 8237 Growth with opalescent zone (+ve reaction)
Escherichia coli NCTC 12241 Growth with no zone (-ve reaction)